Manual of Methods for General Bacteriology |
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Page 89
... nitrogen sources ( glutamate or peptone ) . ' Requires vitamin B12 ( 40 μg / liter ) instead of biotin . m C ... nitrogen - deficient media , these microorganisms fix atmospheric nitrogen ; a trace of molybdate is necessary for nitrogen ...
... nitrogen sources ( glutamate or peptone ) . ' Requires vitamin B12 ( 40 μg / liter ) instead of biotin . m C ... nitrogen - deficient media , these microorganisms fix atmospheric nitrogen ; a trace of molybdate is necessary for nitrogen ...
Page 214
... nitrogen feasible to use , especially when one considers the cost of labor . The cost of liquid nitrogen varies from 20 to 50 cents per liter . A variety of liquid nitrogen refrigerators are now commercially available , with a wide ...
... nitrogen feasible to use , especially when one considers the cost of labor . The cost of liquid nitrogen varies from 20 to 50 cents per liter . A variety of liquid nitrogen refrigerators are now commercially available , with a wide ...
Page 429
... nitrogen gas through sterile culture media in tubes , and in- oculate the media while the tubes are being flushed with nitrogen . Seal the tubes with sterile serum bottle stoppers . Strictly anaerobic condi- tions may not be required ...
... nitrogen gas through sterile culture media in tubes , and in- oculate the media while the tubes are being flushed with nitrogen . Seal the tubes with sterile serum bottle stoppers . Strictly anaerobic condi- tions may not be required ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes