Manual of Methods for General Bacteriology |
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Page 230
... plates . 1. Measure 10 ml of autoclaved nutrient agar ( 13.9.12 ) into sterile petri plates 100 mm in di- ameter , and allow to cool with one edge elevated so that the agar level just reaches the intersec- tion of the bottom and side of ...
... plates . 1. Measure 10 ml of autoclaved nutrient agar ( 13.9.12 ) into sterile petri plates 100 mm in di- ameter , and allow to cool with one edge elevated so that the agar level just reaches the intersec- tion of the bottom and side of ...
Page 236
... plates at 30 ° C for about 18 h or until transductant colonies are just visible to the naked eye . 7. Replica plate to either spectinomycin or streptomycin plates ( 13.9.19 ) and to minimal agar plates ( 13.9.10 ) containing 0.3 ...
... plates at 30 ° C for about 18 h or until transductant colonies are just visible to the naked eye . 7. Replica plate to either spectinomycin or streptomycin plates ( 13.9.19 ) and to minimal agar plates ( 13.9.10 ) containing 0.3 ...
Page 427
... plates . For example , perform the indole test by adding 2 drops of Kovacs ' reagent to the well cultures , or for the Voges - Proskauer test add one loop of a - naphthol followed by one loop ... plate . Nine submaster plates can be prepared.
... plates . For example , perform the indole test by adding 2 drops of Kovacs ' reagent to the well cultures , or for the Voges - Proskauer test add one loop of a - naphthol followed by one loop ... plate . Nine submaster plates can be prepared.
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes