Manual of Methods for General Bacteriology |
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Page 332
Wash Poly - B - hydroxybutyrate , if present , can be exthe test tube , and filter
twice with 2 ml of ice - tracted with chloroform - methanol ( 2 : 1 , vol / vol ) cold 10
% trichloroacetic acid and once with 3 ml at 60°C after the ether - ethanol washes
.
Wash Poly - B - hydroxybutyrate , if present , can be exthe test tube , and filter
twice with 2 ml of ice - tracted with chloroform - methanol ( 2 : 1 , vol / vol ) cold 10
% trichloroacetic acid and once with 3 ml at 60°C after the ether - ethanol washes
.
Page 370
Among these general When an enzyme is thought to be present in
considerations are the following . a crude extract but the activity cannot be
detected , a number of possibilities need to be recVariable assay conditions .
ognized . First , there is ...
Among these general When an enzyme is thought to be present in
considerations are the following . a crude extract but the activity cannot be
detected , a number of possibilities need to be recVariable assay conditions .
ognized . First , there is ...
Page 390
Because of the is obtained if all corrections and controls are small amounts of
end products present in only properly observed . 5 to 10 ml of culture fluid , the
use of a cold A typical pattern of nongaseous end products carrier mixture is ...
Because of the is obtained if all corrections and controls are small amounts of
end products present in only properly observed . 5 to 10 ml of culture fluid , the
use of a cold A typical pattern of nongaseous end products carrier mixture is ...
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Contents
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
GROWTH RALPH N COSTILOW Editor | 63 |
Copyright | |
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Common terms and phrases
absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enzyme example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter Microbiol microscopy mixture mutants needed obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York
Bibliographic information
| Title | Manual of Methods for General Bacteriology |
| Authors | Philipp Gerhardt, American Society for Microbiology |
| Editor | Philipp Gerhardt |
| Edition | illustrated |
| Publisher | American Society for Microbiology, 1981 |
| Original from | the University of Michigan |
| Digitized | 17 Jul 2008 |
| ISBN | 0914826301, 9780914826309 |
| Length | 524 pages |
| Subjects | › Science / General |
| Export Citation | BiBTeX EndNote RefMan |