Manual of Methods for General Bacteriology |
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Page 338
... Procedure . Adjust sample volumes to 0.5 ml . Place in an ice bath . Add 2.25 ml of the sulfuric acid reagent to samples . Mix the tubes in the ice bath for 3 min . Transfer to a 20 ° C water bath for 3 min ; then heat in boiling water ...
... Procedure . Adjust sample volumes to 0.5 ml . Place in an ice bath . Add 2.25 ml of the sulfuric acid reagent to samples . Mix the tubes in the ice bath for 3 min . Transfer to a 20 ° C water bath for 3 min ; then heat in boiling water ...
Page 455
... procedure is that the RNA species are degraded to such an extent that they will not compete with DNA for adsorption sites on the hydroxyl- apatite . For some groups of organisms , RNase T1 may be required in addition to the pancreatic ...
... procedure is that the RNA species are degraded to such an extent that they will not compete with DNA for adsorption sites on the hydroxyl- apatite . For some groups of organisms , RNase T1 may be required in addition to the pancreatic ...
Page 470
... procedure cpm 1,000 TABLE 6. Composition of reaction mixtures used in competition procedure. fractionated . Isolate total RNA , and separate the RNA components by polyacrylamide gel electrophoresis ( 8 , 24 , 40 , 53 ) or by sucrose gra ...
... procedure cpm 1,000 TABLE 6. Composition of reaction mixtures used in competition procedure. fractionated . Isolate total RNA , and separate the RNA components by polyacrylamide gel electrophoresis ( 8 , 24 , 40 , 53 ) or by sucrose gra ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes