Manual of methods for general bacteriology: by Philipp Gerhardt, editor-in-chief ; R.G.E. Murray, editor, I. Morphology ... [et al.]. |
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Page 277
SI endonuclease reaction. SI endonuclease of very good activity can be
purchased commercially (Sigma catalog no. N- 5255). 1. Prepare stock solution
of SI reaction mixture (0.125 mM ZnSO4, 87.5 mM NaCl, 37.5 mM sodium acetate
buffer at ...
SI endonuclease reaction. SI endonuclease of very good activity can be
purchased commercially (Sigma catalog no. N- 5255). 1. Prepare stock solution
of SI reaction mixture (0.125 mM ZnSO4, 87.5 mM NaCl, 37.5 mM sodium acetate
buffer at ...
Page 328
Total Carbohydrates by Anthrone Reaction 333 .2. Total Carbohydrates by
Phenol Reaction 333 .3. D-Glucose 334 .4. Galactose 334 .5. Glycogen 334 .6.
Hexosamines in Purified Peptidoglycans 335 .7. Hexosamines in Complex
Solutions ...
Total Carbohydrates by Anthrone Reaction 333 .2. Total Carbohydrates by
Phenol Reaction 333 .3. D-Glucose 334 .4. Galactose 334 .5. Glycogen 334 .6.
Hexosamines in Purified Peptidoglycans 335 .7. Hexosamines in Complex
Solutions ...
Page 375
The reaction is started with the addition of phosphofructokinase (containing no
more than ca. 10 mil of enzyme) and is followed by measuring the decrease in
optical density at 340 nm. A blank without GTP must be included in each
experiment, ...
The reaction is started with the addition of phosphofructokinase (containing no
more than ca. 10 mil of enzyme) and is followed by measuring the decrease in
optical density at 340 nm. A blank without GTP must be included in each
experiment, ...
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Contents
Electron Microscopy Roger M CoLe anD Terry J Popkin | 34 |
Cell Fractionation CarL A Schnaitman | 52 |
GROWTH RaLph N CostiLow Editor | 65 |
Copyright | |
5 other sections not shown
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Common terms and phrases
acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction g Distilled glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen Pasteur pipette pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove riboflavin salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vol/vol volume Wash wt/vol yeast extract
Bibliographic information
| Title | Manual of methods for general bacteriology: by Philipp Gerhardt, editor-in-chief ; R.G.E. Murray, editor, I. Morphology ... [et al.]. |
| Author | American Society for Microbiology |
| Editors | Philipp Gerhardt, American Society for Microbiology |
| Edition | illustrated, reprint |
| Publisher | American Society for Microbiology, 1981 |
| Original from | the University of Michigan |
| Digitized | 17 Jul 2008 |
| ISBN | 0914826301, 9780914826309 |
| Length | 524 pages |
| Subjects | › › Science / General Science / Life Sciences / Microbiology |
| Export Citation | BiBTeX EndNote RefMan |