Manual of Methods for General Bacteriology |
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Page 31
... solution consisting of 2 g of potassium metabisulfite and 5 ml of concentrated hydrochloric acid in 500 ml of distilled water . 8. Wash the slide with tap water , and counter- stain with a 0.002 % ( wt / vol ) aqueous solution of ...
... solution consisting of 2 g of potassium metabisulfite and 5 ml of concentrated hydrochloric acid in 500 ml of distilled water . 8. Wash the slide with tap water , and counter- stain with a 0.002 % ( wt / vol ) aqueous solution of ...
Page 135
... solution ( sterilized by filtra- tion ) to give a final concentration of 0.5 g of NaHCO3 per liter . Trace elements solution : ZnSO4.7H2O Yeast extract Diammonium hydrogen citrate Tween 80 Distilled water Solution C : MnCl2.4H2O H3BO3 ...
... solution ( sterilized by filtra- tion ) to give a final concentration of 0.5 g of NaHCO3 per liter . Trace elements solution : ZnSO4.7H2O Yeast extract Diammonium hydrogen citrate Tween 80 Distilled water Solution C : MnCl2.4H2O H3BO3 ...
Page 439
... Solution A ( see Caution ) : N- ( 1 - Naphthyl ) -ethylenediamine dihydrochloride ( Sigma Chemi- cal Co. ) HCl , 1.5 N solution 0.02 g 100 ml Dissolve by gentle heating in a fume hood . Solution B : Sulfanilic acid HCl , 1.5 N solution ...
... Solution A ( see Caution ) : N- ( 1 - Naphthyl ) -ethylenediamine dihydrochloride ( Sigma Chemi- cal Co. ) HCl , 1.5 N solution 0.02 g 100 ml Dissolve by gentle heating in a fume hood . Solution B : Sulfanilic acid HCl , 1.5 N solution ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes