Manual of Methods for General Bacteriology |
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Page 8
... specimen , which must then be improved by adjusting the position of the condenser and the aperture diaphragm for the best image . 3. In our experience , the best way to deter- mine the optimum condenser position is to re- move the ...
... specimen , which must then be improved by adjusting the position of the condenser and the aperture diaphragm for the best image . 3. In our experience , the best way to deter- mine the optimum condenser position is to re- move the ...
Page 11
... specimen ( i.e. , objective and condenser are now both in focus ) . Then readjust the field diaphragm so that its image just impinges on the field of view . Note : If the margins of the field diaphragm give an uneven color fringe when ...
... specimen ( i.e. , objective and condenser are now both in focus ) . Then readjust the field diaphragm so that its image just impinges on the field of view . Note : If the margins of the field diaphragm give an uneven color fringe when ...
Page 35
... specimen preparation ) . But biolog- ical specimens are not infinitely thin ; the prac- tical limit of real resolution in sections is about 2.5 nm , and in negatively stained preparations it is about 1.0 nm . It takes skill and a well ...
... specimen preparation ) . But biolog- ical specimens are not infinitely thin ; the prac- tical limit of real resolution in sections is about 2.5 nm , and in negatively stained preparations it is about 1.0 nm . It takes skill and a well ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes