Manual of Methods for General Bacteriology |
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Page 53
... spheroplasts or protoplasts in osmotically stabilized medium are employed , it is essential to determine the per- centage of conversion in a counting chamber rather than simply to examine wet mounts . This is because lysed spheroplasts ...
... spheroplasts or protoplasts in osmotically stabilized medium are employed , it is essential to determine the per- centage of conversion in a counting chamber rather than simply to examine wet mounts . This is because lysed spheroplasts ...
Page 55
... spheroplasts instead of protoplasts . Spheroplasts of enteric bacteria can be pre- pared by the procedure of Osborn and Munson ( 8 ) , as follows . Cultures are centrifuged and sus- pended in one - tenth of the culture volume in 55 cold ...
... spheroplasts instead of protoplasts . Spheroplasts of enteric bacteria can be pre- pared by the procedure of Osborn and Munson ( 8 ) , as follows . Cultures are centrifuged and sus- pended in one - tenth of the culture volume in 55 cold ...
Page 403
... Spheroplasts of gram - negative bacteria such as E. coli can be prepared by the lysozyme - ethyl- enediaminetetraacetate method ( 18 ) , which leaves the outer membrane of the cell wall as- sociated with the spheroplast . Protoplasts of ...
... Spheroplasts of gram - negative bacteria such as E. coli can be prepared by the lysozyme - ethyl- enediaminetetraacetate method ( 18 ) , which leaves the outer membrane of the cell wall as- sociated with the spheroplast . Protoplasts of ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes