Manual of Methods for General Bacteriology |
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Page 139
0 with sterile H2SO4 . For a solid medium , Deionized water 1 , 000 ml add 15 g
of agar per liter before adjusting the pH . Boil to dissolve the agar , sterilize by
auto8 . 5 . 56 . Violet Red Bile Agar ( 6 ) ( BBL or claving , and cool to 45 to 50°C
...
0 with sterile H2SO4 . For a solid medium , Deionized water 1 , 000 ml add 15 g
of agar per liter before adjusting the pH . Boil to dissolve the agar , sterilize by
auto8 . 5 . 56 . Violet Red Bile Agar ( 6 ) ( BBL or claving , and cool to 45 to 50°C
...
Page 209
Immersing in Mineral Oil Many bacterial species can be successfully preserved
for months or years by the relatively simple and cheap method of immersing in
sterile medicinal - grade mineral oil ( paraffin oil of specific gravity of 0 . 865 to 0 .
Immersing in Mineral Oil Many bacterial species can be successfully preserved
for months or years by the relatively simple and cheap method of immersing in
sterile medicinal - grade mineral oil ( paraffin oil of specific gravity of 0 . 865 to 0 .
Page 214
DMSO is filter sterilized by 200°C ( + 0 . 3°C ) . The unit is battery powered using
03 porosity Selas filter candles , collected and can be mounted on a wall . in 10 -
to 15 - ml quantities in sterile test tubes , and then stored in the frozen state at ...
DMSO is filter sterilized by 200°C ( + 0 . 3°C ) . The unit is battery powered using
03 porosity Selas filter candles , collected and can be mounted on a wall . in 10 -
to 15 - ml quantities in sterile test tubes , and then stored in the frozen state at ...
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Contents
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
GROWTH RALPH N COSTILOW Editor | 63 |
Copyright | |
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Common terms and phrases
absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enzyme example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter Microbiol microscopy mixture mutants needed obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York
Bibliographic information
| Title | Manual of Methods for General Bacteriology |
| Author | Philipp Gerhardt |
| Editor | Philipp Gerhardt |
| Contributors | R. G. E. Murray, Ralph N. Costilow, American Society for Microbiology, Willis A. Wood |
| Edition | illustrated, reprint |
| Publisher | American Society for Microbiology, 1981 |
| Original from | the University of Michigan |
| Digitized | 17 Jul 2008 |
| ISBN | 0914826298, 9780914826293 |
| Length | 524 pages |
| Export Citation | BiBTeX EndNote RefMan |