Manual of Methods for General Bacteriology |
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Page 139
Adjust to pH 3.5 to 4.0 with sterile H2SO4 . For a solid medium , add 15 g of aga per liter before adjusting the pH . Boil to dissolve the agar , sterilize by autoclaving , and cool to 45 to 50 ° C . Adjust pH to 3.5 to 4.0 with sterile ...
Adjust to pH 3.5 to 4.0 with sterile H2SO4 . For a solid medium , add 15 g of aga per liter before adjusting the pH . Boil to dissolve the agar , sterilize by autoclaving , and cool to 45 to 50 ° C . Adjust pH to 3.5 to 4.0 with sterile ...
Page 209
Sporeforming bacteria have been successfully preserved for years by use of a mixture of sterile , 12.1.2 . Immersing in Mineral Oil air - dried soil ( 10 ) . Sterilize the soil by autoclavMany bacterial species can be successfullying ...
Sporeforming bacteria have been successfully preserved for years by use of a mixture of sterile , 12.1.2 . Immersing in Mineral Oil air - dried soil ( 10 ) . Sterilize the soil by autoclavMany bacterial species can be successfullying ...
Page 264
The powder is weighed out , suspended in sterile water , and added to media to the desired final concentration . Alternatively , stock solutions can be prepared and stored at -20 ° C . Corrections should be made for buffers or other ...
The powder is weighed out , suspended in sterile water , and added to media to the desired final concentration . Alternatively , stock solutions can be prepared and stored at -20 ° C . Corrections should be made for buffers or other ...
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Contents
1 | 22 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino amount anaerobic applications appropriate assay autoclaving bacteria base broth buffer cells centrifuge chemical colonies column components concentration containing counting cover culture cytoplasmic described determine dilution Dissolve distilled water drop effective electron enzyme examination example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method microscopy mixture mutants needed objective obtained organisms oxygen plasmid plate positive possible prepared present Press procedure protein reaction reagent references remove sample selection separation slide sodium solution specific specimen staining standard sterile surface suspension Table techniques temperature tion transfer tube usually volume Wash York