Manual of Methods for General Bacteriology |
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Page 41
... suspension or culture . 2. Wash the pellet by suspension in fresh cul- ture medium or Veronal - acetate buffer , centri- fuge , and suspend in tryptone medium or tryptic soy broth . ( The use of tryptone or tryptic soy broth is optional ...
... suspension or culture . 2. Wash the pellet by suspension in fresh cul- ture medium or Veronal - acetate buffer , centri- fuge , and suspend in tryptone medium or tryptic soy broth . ( The use of tryptone or tryptic soy broth is optional ...
Page 53
... suspension ( 5 to 40 ml of a suspension , up to 30 % cells by volume ) is placed in a steel cylinder fitted with a piston and a small relief valve connected to an outlet tube . This entire assembly is placed in a 10 - ton hy- draulic ...
... suspension ( 5 to 40 ml of a suspension , up to 30 % cells by volume ) is placed in a steel cylinder fitted with a piston and a small relief valve connected to an outlet tube . This entire assembly is placed in a 10 - ton hy- draulic ...
Page 369
... suspensions of cells can be used , breakage efficiency is decreased considerably in viscous solutions . As large mol- ecules such as deoxyribonucleic acid are released from broken cells , viscosity of the suspension increases and a ...
... suspensions of cells can be used , breakage efficiency is decreased considerably in viscous solutions . As large mol- ecules such as deoxyribonucleic acid are released from broken cells , viscosity of the suspension increases and a ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes