Manual of Methods for General Bacteriology |
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Page 29
... ( wt / vol ) aqueous solution of crystal violet . 2. Wash off the dye with a 20 % ( wt / vol ) aqueous solution of copper sulfate ( CuSO4 . 5H2O ) , drain , and blot dry . Capsules appear light blue , and organisms are dark purple . 3.3 ...
... ( wt / vol ) aqueous solution of crystal violet . 2. Wash off the dye with a 20 % ( wt / vol ) aqueous solution of copper sulfate ( CuSO4 . 5H2O ) , drain , and blot dry . Capsules appear light blue , and organisms are dark purple . 3.3 ...
Page 30
... ( vol / vol ) alcohol are not recom- mended for cleaning slides to be used for staining flagella . Leifson method for ... ( wt / vol ) Sudan black B made up in ethylene glycol . Filter and stain for 5 to 15 min ( determine time ...
... ( vol / vol ) alcohol are not recom- mended for cleaning slides to be used for staining flagella . Leifson method for ... ( wt / vol ) Sudan black B made up in ethylene glycol . Filter and stain for 5 to 15 min ( determine time ...
Page 32
... ( wt / vol ) aqueous solution of malachite green oxalate also is required . Procedure : generally rod shaped , occur in pairs , and show marked pleomorphism . Rickettsiae may be seen in films of infected tissue by direct microscopic ...
... ( wt / vol ) aqueous solution of malachite green oxalate also is required . Procedure : generally rod shaped , occur in pairs , and show marked pleomorphism . Rickettsiae may be seen in films of infected tissue by direct microscopic ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer CaCl2 calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes