Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 166
... first type of experiment . This is the same as setting этз = am3 It has been shown ( Stigter , 1960b ) that the error resulting from this approximation is negligibly small . While on first inspection it might ap- pear that the ...
... first type of experiment . This is the same as setting этз = am3 It has been shown ( Stigter , 1960b ) that the error resulting from this approximation is negligibly small . While on first inspection it might ap- pear that the ...
Page 174
... first dialyze all the protein solutions against the solvent and use the dialyzate as reference solvent . A series of light - scattering experiments must be carried out in order to ob- tain M2 , M4 , B22 , and ẞ44 . These experiments ...
... first dialyze all the protein solutions against the solvent and use the dialyzate as reference solvent . A series of light - scattering experiments must be carried out in order to ob- tain M2 , M4 , B22 , and ẞ44 . These experiments ...
Page 310
... first - order helix - formation shift ( A ) which is also downfield ( Section IV , C ) . First - order shifts resulting from changes in the primary or secondary structure are only observed for the CaH2 protons , so that if large ...
... first - order helix - formation shift ( A ) which is also downfield ( Section IV , C ) . First - order shifts resulting from changes in the primary or secondary structure are only observed for the CaH2 protons , so that if large ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone