Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 340
... RNase A in deutero- acetate buffer at various pH values . Peaks 1-4 are C - 2 imidazole peaks of the four histidine ... RNase , rather little detailed information has been obtained about partially unfolded conformations . Some of the ...
... RNase A in deutero- acetate buffer at various pH values . Peaks 1-4 are C - 2 imidazole peaks of the four histidine ... RNase , rather little detailed information has been obtained about partially unfolded conformations . Some of the ...
Page 342
... RNase A at pH 5.37 in the presence of inhibitors . RNase A alone ; RNase A + 0.001 M 3 ' - CMP ; RNase A + 0.002 M 5 ' - CMP ( Meadows et al . , 1967 ) . elegant study of RNase histidine derivatives by Jardetzky and Scheraga and their ...
... RNase A at pH 5.37 in the presence of inhibitors . RNase A alone ; RNase A + 0.001 M 3 ' - CMP ; RNase A + 0.002 M 5 ' - CMP ( Meadows et al . , 1967 ) . elegant study of RNase histidine derivatives by Jardetzky and Scheraga and their ...
Page 343
... RNase S ' when compared with RNase S allowed the missing His - 12 proton to be assigned to peak 2 and therefore His - 112 to peak 3. The anomalous chemical shift in the acid pH region of peak 4 ( Fig . 33 ) , and its broad line width in ...
... RNase S ' when compared with RNase S allowed the missing His - 12 proton to be assigned to peak 2 and therefore His - 112 to peak 3. The anomalous chemical shift in the acid pH region of peak 4 ( Fig . 33 ) , and its broad line width in ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone