Physical Principles and Techniques of Protein Chemistry, Part 2Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 230
1 % ) are required to produce adequate absorption due to the protein . Further
difficulties are encountered if a second cell is introduced into the reference beam
to compensate for the water absorption . The compensating cell must have ...
1 % ) are required to produce adequate absorption due to the protein . Further
difficulties are encountered if a second cell is introduced into the reference beam
to compensate for the water absorption . The compensating cell must have ...
Page 277
The observation of nuclear magnetic resonance depends on the absorption of
radiofrequency radiation by nuclei with magnetic moments oriented in a strong
magnetic field ; the absorption of energy results in the reorientation of nuclei with
...
The observation of nuclear magnetic resonance depends on the absorption of
radiofrequency radiation by nuclei with magnetic moments oriented in a strong
magnetic field ; the absorption of energy results in the reorientation of nuclei with
...
Page 479
Subject Index metal ions interacting with , 309 Absorption coefficient , definition of
, 227 pH - dependence of chemical shifts of , pH - dependence Absorption of light
, effect on light scat306 , 307 tering , 177 Amino groups , see also Lysyl side ...
Subject Index metal ions interacting with , 309 Absorption coefficient , definition of
, 227 pH - dependence of chemical shifts of , pH - dependence Absorption of light
, effect on light scat306 , 307 tering , 177 Amino groups , see also Lysyl side ...
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Contents
LIST OF CONTRIBUTORS | 1 |
Zonal Analytical Velocity Sedimentation | 2 |
Light Scattering | 12 |
Copyright | |
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absorption acid applied associated average band beam binding boundary calculated cell centrifugal Chem chemical shifts column complex component concentration constant correction density density gradient dependence described determined diffusion distance distribution effect equal equation equilibrium error et al experiments factor field frequency fringe function give given gradient groups increase interaction length light macromolecule magnetic maximum means measured meniscus method molecular weight molecule necessary observed obtained occur optical particle pattern peak photographic Phys plate plot Polymer position possible procedure protein protein solution protons range ratio reference refractive region relatively resonance rotation rotor salt sample scattering schlieren sector sedimentation sedimentation coefficient shape shown shows single slit solution solvent specific spectra spectrum speed structure studies technique temperature tion ultracentrifuge usually values viscosity volume zero zone