Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 127
... acid and stronger organic acids . Thus the polyelectrolyte effect has been observed for poly- amides dissolved in formic acid ( Schaefgen and Trivisonno , 1951 , 1952 ; Saunders , 1962 , 1964 ) , poly - L - amino acids in mixtures of ...
... acid and stronger organic acids . Thus the polyelectrolyte effect has been observed for poly- amides dissolved in formic acid ( Schaefgen and Trivisonno , 1951 , 1952 ; Saunders , 1962 , 1964 ) , poly - L - amino acids in mixtures of ...
Page 139
... acid or trifluoroacetic acid to the helicogenic . solvent . This is shown by the fact that PBLG in 80 % ethylene dichloride- 20 % dichloroacetic acid gives a polyelectrolyte effect ( Section V , C ) , which is also unequivocal proof ...
... acid or trifluoroacetic acid to the helicogenic . solvent . This is shown by the fact that PBLG in 80 % ethylene dichloride- 20 % dichloroacetic acid gives a polyelectrolyte effect ( Section V , C ) , which is also unequivocal proof ...
Page 140
... acids which are soluble in organic solvents or for those soluble in water ( e.g. , poly - L - glutamic acid ) , is promoted by electrostatic repulsion of fixed charges on the peptide groups in the former case ( Brad- bury and Fenn ...
... acids which are soluble in organic solvents or for those soluble in water ( e.g. , poly - L - glutamic acid ) , is promoted by electrostatic repulsion of fixed charges on the peptide groups in the former case ( Brad- bury and Fenn ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone