Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 86
... band center at 1 atm pressure can be calculated from the approximate relationship of Eq . ( 69 ) Po = w2x 。 Pe + ... band center . Values of ßo for a range of useful gradient - forming salts and densities are given by Ifft et al ...
... band center at 1 atm pressure can be calculated from the approximate relationship of Eq . ( 69 ) Po = w2x 。 Pe + ... band center . Values of ßo for a range of useful gradient - forming salts and densities are given by Ifft et al ...
Page 89
... band of macro- solute and is determined from either schlieren or absorption data . In the case of a schlieren trace ( Fig . 30 ) , the center of the band is taken as the point of intersection of the biphasic trace due to the solute band ...
... band of macro- solute and is determined from either schlieren or absorption data . In the case of a schlieren trace ( Fig . 30 ) , the center of the band is taken as the point of intersection of the biphasic trace due to the solute band ...
Page 241
... Band Intensities The evaluation of the area under an absorption band has been discussed in detail by Ramsay ( 1952 ) and Seshadri and Jones ( 1963 ) . This area may be used to calculate the integrated intensity and hence the mag- nitude ...
... Band Intensities The evaluation of the area under an absorption band has been discussed in detail by Ramsay ( 1952 ) and Seshadri and Jones ( 1963 ) . This area may be used to calculate the integrated intensity and hence the mag- nitude ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone