Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 185
... cell . Each small cell , too , due to unavoidable manufacturing tolerances , is slightly different . Sometimes it is a trifle out of square , for example , so that individual small cells used must be standardized separately . We have ...
... cell . Each small cell , too , due to unavoidable manufacturing tolerances , is slightly different . Sometimes it is a trifle out of square , for example , so that individual small cells used must be standardized separately . We have ...
Page 186
... cell 30 - ml cell 1 6.0 10.5 15.0 30.0 60.0 120.0 Fluorescein concentration ( gm / liter x 106 ) O 240.0 FIG . 11. Typical calibration data for 1 cm square cells , using fluorescein over a 160 - fold concentration range ( from Timasheff ...
... cell 30 - ml cell 1 6.0 10.5 15.0 30.0 60.0 120.0 Fluorescein concentration ( gm / liter x 106 ) O 240.0 FIG . 11. Typical calibration data for 1 cm square cells , using fluorescein over a 160 - fold concentration range ( from Timasheff ...
Page 196
... cells are generally useful to within 30 ° of the exit beam ; below this angle internal reflections become unavoidably large . This cell , in common with most light - scattering cells used in the second generation of light - scattering ...
... cells are generally useful to within 30 ° of the exit beam ; below this angle internal reflections become unavoidably large . This cell , in common with most light - scattering cells used in the second generation of light - scattering ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone