Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
From inside the book
Results 1-3 of 20
Page 5
... centrifugal field of about 250,000 g , corresponding to almost 60,000 rpm for a conventional ultracentrifuge rotor . Under this acceleration the solute protein molecules move with a steady terminal velocity , along a radial path through ...
... centrifugal field of about 250,000 g , corresponding to almost 60,000 rpm for a conventional ultracentrifuge rotor . Under this acceleration the solute protein molecules move with a steady terminal velocity , along a radial path through ...
Page 11
... centrifugal field such that the rate of sedimentation of the pro- tein molecules away from the center of rotation is of the same order as the rate of diffusion of molecules from high concentration regions near the cell bottom to the ...
... centrifugal field such that the rate of sedimentation of the pro- tein molecules away from the center of rotation is of the same order as the rate of diffusion of molecules from high concentration regions near the cell bottom to the ...
Page 94
... centrifugal force similar to the M.S.E. machine . The rotor speed is electronically controlled and is measured to an accuracy of ± 10 rpm . The rotor can be thermostated at temperatures between 0 ° and 30 ° C and rotates in a hydrogen ...
... centrifugal force similar to the M.S.E. machine . The rotor speed is electronically controlled and is measured to an accuracy of ± 10 rpm . The rotor can be thermostated at temperatures between 0 ° and 30 ° C and rotates in a hydrogen ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
51 other sections not shown
Other editions - View all
Common terms and phrases
absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone