Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 16
... CHARGE 1. Velocity Sedimentation : Primary and Secondary Salt Effects If a charged protein is sedimented in the absence of added low molec- ular weight electrolyte , because of the strong electrostatic coupling be- tween the protein and ...
... CHARGE 1. Velocity Sedimentation : Primary and Secondary Salt Effects If a charged protein is sedimented in the absence of added low molec- ular weight electrolyte , because of the strong electrostatic coupling be- tween the protein and ...
Page 127
... charge on the molecule and it expands on dilution . However , in the region of the isoelectric pH ( the pH at which the net charge on the molecule is zero ) the electrostatic attractive forces within the molecule can cause the opposite ...
... charge on the molecule and it expands on dilution . However , in the region of the isoelectric pH ( the pH at which the net charge on the molecule is zero ) the electrostatic attractive forces within the molecule can cause the opposite ...
Page 174
... charge of the protein in protonic units e , ‹ is the dielectric constant of the medium , k is Boltzmann's constant , κ and a are the Debye - Hückel parameters , NA is Avogadro's number , ( Z2 ) av is the average net charge of the ...
... charge of the protein in protonic units e , ‹ is the dielectric constant of the medium , k is Boltzmann's constant , κ and a are the Debye - Hückel parameters , NA is Avogadro's number , ( Z2 ) av is the average net charge of the ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone