Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 45
... Column , 1 mm Column , Zero Meniscus Concentration ( High Speed ) Methods a . 3 mm Column ( Van Holde and Baldwin , 1958 ) . In this method a column of solution 3 mm long with a volume of about 0.1 ml ( Fig . 16 ) and a concentration of ...
... Column , 1 mm Column , Zero Meniscus Concentration ( High Speed ) Methods a . 3 mm Column ( Van Holde and Baldwin , 1958 ) . In this method a column of solution 3 mm long with a volume of about 0.1 ml ( Fig . 16 ) and a concentration of ...
Page 48
... column is always very slightly shorter than the solvent column as shown in Fig . 16 , so that the complete solution column is observable . Where several cells are run simultaneously , care must be taken to avoid the air fringes of the ...
... column is always very slightly shorter than the solvent column as shown in Fig . 16 , so that the complete solution column is observable . Where several cells are run simultaneously , care must be taken to avoid the air fringes of the ...
Page 87
... column height is greater than that of the solution ; when the cell is run in the centrifuge , solvent will be transferred into the solution sector until the column heights are iden- tical . In this way problems caused by differential ...
... column height is greater than that of the solution ; when the cell is run in the centrifuge , solvent will be transferred into the solution sector until the column heights are iden- tical . In this way problems caused by differential ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone