Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 60
... compared with 1 atm at the upper meniscus . For a reaction with a AV of reaction of about +800 cm3 mole - 1 , the ratio of the equilibrium constants at the top to that at the bottom of the cell would be 0.25 X 10-5 for a solution column ...
... compared with 1 atm at the upper meniscus . For a reaction with a AV of reaction of about +800 cm3 mole - 1 , the ratio of the equilibrium constants at the top to that at the bottom of the cell would be 0.25 X 10-5 for a solution column ...
Page 296
... compared with the Larmor period ( 1 / 2wo 10-8 sec ) . This is the " extreme narrowing " condition ( Te << ( 2πωο 1/270 ) under which sharp resonances are observed . The main features of resonance were shown by Bloembergen et al ...
... compared with the Larmor period ( 1 / 2wo 10-8 sec ) . This is the " extreme narrowing " condition ( Te << ( 2πωο 1/270 ) under which sharp resonances are observed . The main features of resonance were shown by Bloembergen et al ...
Page 353
... compared the relative intensities of the upfield resonances in the 220 MHz spectra of McDonald and Phillips ( 1967 ) with optical rotatory dispersion ( ORD ) measurements at 233 mμ over the same temperature ranges . Both the PMR and ORD ...
... compared the relative intensities of the upfield resonances in the 220 MHz spectra of McDonald and Phillips ( 1967 ) with optical rotatory dispersion ( ORD ) measurements at 233 mμ over the same temperature ranges . Both the PMR and ORD ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone