Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 184
... containing a protein in reasonable concentration ( plus allow- ance for volume losses during clarification ) ... contain over five dollars ' worth of B - grade protein . We have adopted the small - cell method developed by Dintzis ( Tima ...
... containing a protein in reasonable concentration ( plus allow- ance for volume losses during clarification ) ... contain over five dollars ' worth of B - grade protein . We have adopted the small - cell method developed by Dintzis ( Tima ...
Page 374
... containing the protein and the other not ( Klotz et al . , 1946 ) . In these methods an indifferent electrolyte is included whenever possible to allow for Donnan effects and to swamp out any in- fluence of the protein on the activity of ...
... containing the protein and the other not ( Klotz et al . , 1946 ) . In these methods an indifferent electrolyte is included whenever possible to allow for Donnan effects and to swamp out any in- fluence of the protein on the activity of ...
Page 387
... containing K2HgI4 . In such a system any of the four species HgI42 , HgI3 , HgI2 , and I may be bound . Donor nitrogen atoms in imidazole or amino groups in the protein may displace iodide ions from the HgI2- ion ( Bluhm et al . , 1958 ) ...
... containing K2HgI4 . In such a system any of the four species HgI42 , HgI3 , HgI2 , and I may be bound . Donor nitrogen atoms in imidazole or amino groups in the protein may displace iodide ions from the HgI2- ion ( Bluhm et al . , 1958 ) ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone