Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 36
... determination of molecular weight by the equilibrium method . The species whose molecular weight is determined is defined by the con- centration units used in the evaluation of the density increment or partial specific volume ( Section ...
... determination of molecular weight by the equilibrium method . The species whose molecular weight is determined is defined by the con- centration units used in the evaluation of the density increment or partial specific volume ( Section ...
Page 79
... determined by the ultracen- trifuge method refers to the sedimenting species whose composition is determined by the solvent in which it sediments . Solvation both by water and by the solute added to increase the density of the solution ...
... determined by the ultracen- trifuge method refers to the sedimenting species whose composition is determined by the solvent in which it sediments . Solvation both by water and by the solute added to increase the density of the solution ...
Page 89
... determined and may be calculated as the product of the experimentally determined buoyancy density gradient ( Eq . 73 ) and the factor ( 1a ) . a is defined by Eq . ( 76 ) α = дроо da1 ° да1 Р dp0 ( 76 ) Р where ( po / da1 ) is the slope ...
... determined and may be calculated as the product of the experimentally determined buoyancy density gradient ( Eq . 73 ) and the factor ( 1a ) . a is defined by Eq . ( 76 ) α = дроо da1 ° да1 Р dp0 ( 76 ) Р where ( po / da1 ) is the slope ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone