Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 218
... difficulty , the reference beam signal may be monitored continuously and the slit width varied automatically to maintain a suitable level of servosignal per unit comb displacement . This system has the advantage that the spectrum is ...
... difficulty , the reference beam signal may be monitored continuously and the slit width varied automatically to maintain a suitable level of servosignal per unit comb displacement . This system has the advantage that the spectrum is ...
Page 237
... difficulties of interpreting measurements made with unpolarized radiation are discussed in Fraser ( 1960 , p . 322 ) and these difficulties are greatly magnified when grating instruments are used because of their polarizing properties ...
... difficulties of interpreting measurements made with unpolarized radiation are discussed in Fraser ( 1960 , p . 322 ) and these difficulties are greatly magnified when grating instruments are used because of their polarizing properties ...
Page 262
... difficulties can be resolved , a proper anal- ysis of the amide I region is essential because of the very considerable overlap between the various components . The grossly misleading results that may occur from using peak heights to ...
... difficulties can be resolved , a proper anal- ysis of the amide I region is essential because of the very considerable overlap between the various components . The grossly misleading results that may occur from using peak heights to ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone