Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 117
... ellipsoid is a rea- sonable approximation for a rigid rod and Eqs . ( 28 ) and ( 29 ) can be combined in order to obtain p for the ellipsoid . It is assumed that the ellipsoid has the same length 2a and volume as a cylinder of length L ...
... ellipsoid is a rea- sonable approximation for a rigid rod and Eqs . ( 28 ) and ( 29 ) can be combined in order to obtain p for the ellipsoid . It is assumed that the ellipsoid has the same length 2a and volume as a cylinder of length L ...
Page 132
... ellipsoid . Thus it can be said that the particle behaves hydrodynamically as though it were an ellipsoid of effective volume ve and axial ratio p ; indeed , this is the ellipsoid which is equivalent to the real particle . ( 3 ) The ẞ ...
... ellipsoid . Thus it can be said that the particle behaves hydrodynamically as though it were an ellipsoid of effective volume ve and axial ratio p ; indeed , this is the ellipsoid which is equivalent to the real particle . ( 3 ) The ẞ ...
Page 133
... Ellipsoid and String of Spherical Beads If Eq . ( 53 ) is combined with the simple equation for the volume ve of the equivalent prolate ellipsoid , namely , ve = 4ab2 / 3 , then its length 2a becomes 2a = ( 300 / 4πN ) 1 / 3 ( p2 / v ) ...
... Ellipsoid and String of Spherical Beads If Eq . ( 53 ) is combined with the simple equation for the volume ve of the equivalent prolate ellipsoid , namely , ve = 4ab2 / 3 , then its length 2a becomes 2a = ( 300 / 4πN ) 1 / 3 ( p2 / v ) ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone