Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 44
... Equations . The molecular weight of a protein may be obtained from its s ° value alone by means of the equation M = - s ° N6πη [ 3 / 4πN ] 1/2 ( 1 − up ) 1/3 3/2 ( 29 ) only if f / fo equals unity . For most proteins this is not the ...
... Equations . The molecular weight of a protein may be obtained from its s ° value alone by means of the equation M = - s ° N6πη [ 3 / 4πN ] 1/2 ( 1 − up ) 1/3 3/2 ( 29 ) only if f / fo equals unity . For most proteins this is not the ...
Page 101
... equation rotational frictional coefficients of spherical and actual particles function in the equation which combines [ n ] and I. Introduction Among physical techniques used for the determination of size and shape of protein molecules ...
... equation rotational frictional coefficients of spherical and actual particles function in the equation which combines [ n ] and I. Introduction Among physical techniques used for the determination of size and shape of protein molecules ...
Page 105
... equation has a reasonable theoret- ical basis ( Section IV , A ) . On these grounds , and on the basis of current usage , the Huggins equa- tion is to be preferred , but in view of the experimental differences found by Ibrahim ( 1965 ) ...
... equation has a reasonable theoret- ical basis ( Section IV , A ) . On these grounds , and on the basis of current usage , the Huggins equa- tion is to be preferred , but in view of the experimental differences found by Ibrahim ( 1965 ) ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone