Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 204
... factor , defined in Eq . ( 37 ) ( Section III , B , 2 ) . It is the ratio of increment of scattering of a given cell to an absolute cell containing the same clean solution . The last factor on the right - hand side of Eq . ( 38 ) is C ...
... factor , defined in Eq . ( 37 ) ( Section III , B , 2 ) . It is the ratio of increment of scattering of a given cell to an absolute cell containing the same clean solution . The last factor on the right - hand side of Eq . ( 38 ) is C ...
Page 206
... factor by which the beam was diminished in intensity . If neutral filter 2 has a transmittance of 0.2240 G90 8740 1 ... factor F in Eq . ( 38 ) is no longer cancelled by the four filters being in the beam at 0 ° , and the ratio of 90 ...
... factor by which the beam was diminished in intensity . If neutral filter 2 has a transmittance of 0.2240 G90 8740 1 ... factor F in Eq . ( 38 ) is no longer cancelled by the four filters being in the beam at 0 ° , and the ratio of 90 ...
Page 237
... factor of 1.78 , or the amplifier gain ( hence noise ) by a factor of 3.16 . If an increase in noise cannot be tolerated the time constant and scan time must be increased by a factor of 10 . 3. Compensated Spectra When solution spectra ...
... factor of 1.78 , or the amplifier gain ( hence noise ) by a factor of 3.16 . If an increase in noise cannot be tolerated the time constant and scan time must be increased by a factor of 10 . 3. Compensated Spectra When solution spectra ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone