Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 90
... in isolation at 1 atm except at infinite dilution . However , in a density gradient it appears that , depending on the conditions , 90 J. H. COATES Fluorescence of Proteins Raymond F Chen, Harold Edelhoch, and Robert F Steiner.
... in isolation at 1 atm except at infinite dilution . However , in a density gradient it appears that , depending on the conditions , 90 J. H. COATES Fluorescence of Proteins Raymond F Chen, Harold Edelhoch, and Robert F Steiner.
Page 180
... fluorescence , reflectance , depolarization , etc. ) may be carried out , while also considering cost , ease of construction and ad- justment , accuracy , and convenience of operation in one mode . These are decisions which an ...
... fluorescence , reflectance , depolarization , etc. ) may be carried out , while also considering cost , ease of construction and ad- justment , accuracy , and convenience of operation in one mode . These are decisions which an ...
Page 185
... = ( G90 / Go ) fluore fluorescein ( G90 / Go ) w ― water Actually , in the case of fluorescein , it is not scattering , but fluorescence which is viewed . Fluorescein concentration / intensity at 90 ° 7.9 7.6 7.3 12. LIGHT SCATTERING 185.
... = ( G90 / Go ) fluore fluorescein ( G90 / Go ) w ― water Actually , in the case of fluorescein , it is not scattering , but fluorescence which is viewed . Fluorescein concentration / intensity at 90 ° 7.9 7.6 7.3 12. LIGHT SCATTERING 185.
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone