Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 33
... important that the instrument be linear in terms of the optical den- sity of the film ; this should be checked with calibrated neutral density filters . The instrument should also be capable of reasonable resolution . This may be ...
... important that the instrument be linear in terms of the optical den- sity of the film ; this should be checked with calibrated neutral density filters . The instrument should also be capable of reasonable resolution . This may be ...
Page 108
... important factor is the choice of a precision bore capillary of the correct radius . The timing of the movement of the meniscus from the line at A to the line at B is usually measured with a stopwatch , with an error in the flow time of ...
... important factor is the choice of a precision bore capillary of the correct radius . The timing of the movement of the meniscus from the line at A to the line at B is usually measured with a stopwatch , with an error in the flow time of ...
Page 167
... important to point out that the prefer- ential interaction , as measured by light scattering and differential re- fractometry , as well as by some other thermodynamic techniques , is strictly a measurement of changes in activity ...
... important to point out that the prefer- ential interaction , as measured by light scattering and differential re- fractometry , as well as by some other thermodynamic techniques , is strictly a measurement of changes in activity ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone