Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 179
... intensity must always be sacrificed . to improve collimation , or more pertinent to the area of light - scattering photometry , collimation must always be more or less sacrificed to obtain the necessary intensity . It is also a painful ...
... intensity must always be sacrificed . to improve collimation , or more pertinent to the area of light - scattering photometry , collimation must always be more or less sacrificed to obtain the necessary intensity . It is also a painful ...
Page 184
... intensity is measured after it has passed through the cell ( and is actually a transmitted intensity ) , this reflection subtracts intensity from the measured 0 ° beam and must be corrected for . In in- struments where the true incident ...
... intensity is measured after it has passed through the cell ( and is actually a transmitted intensity ) , this reflection subtracts intensity from the measured 0 ° beam and must be corrected for . In in- struments where the true incident ...
Page 303
... intensity , and will lead to an under- estimate of T2 . Operational conditions are optimized to obtain undistorted absorption peaks at the maximum signal - to - noise ratio . For broad sig- nals with short T2 values , higher rf intensities ...
... intensity , and will lead to an under- estimate of T2 . Operational conditions are optimized to obtain undistorted absorption peaks at the maximum signal - to - noise ratio . For broad sig- nals with short T2 values , higher rf intensities ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone