Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 201
... limited to aqueous solutions at room temperature . J. J. Katz ( 1954a , b ) has measured the light scattered from solutions of proteins in anhydrous HF , a most exotic solvent , and the present authors ( Timasheff and Townend , 1961a ) ...
... limited to aqueous solutions at room temperature . J. J. Katz ( 1954a , b ) has measured the light scattered from solutions of proteins in anhydrous HF , a most exotic solvent , and the present authors ( Timasheff and Townend , 1961a ) ...
Page 218
... limited to be of value . Unless there is some visual indication of the condition in which the adjustment required is out- side the range of the automatic slit control , errors may be introduced in an insidious way . c . Automatic Gain ...
... limited to be of value . Unless there is some visual indication of the condition in which the adjustment required is out- side the range of the automatic slit control , errors may be introduced in an insidious way . c . Automatic Gain ...
Page 353
... limited length , and calculations 100 Lysozyme PD = 4.6 80 60 40 20 1 T T 100 80 ORD at 233 mμ 60 NMR integrated intensity 40 0 95 90 85 80 75 70 65 60 55 Temperature ( ° C ) 50 20 FIG . 41. A comparison with optical rotatory dispersion ...
... limited length , and calculations 100 Lysozyme PD = 4.6 80 60 40 20 1 T T 100 80 ORD at 233 mμ 60 NMR integrated intensity 40 0 95 90 85 80 75 70 65 60 55 Temperature ( ° C ) 50 20 FIG . 41. A comparison with optical rotatory dispersion ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone