Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 79
... means of the relation Uh = ( 1 / po ) ( 55 ) In general the hydrodynamic partial specific volume is not identical to the partial specific volume determined by conventional means ( see Part C of this series ) . The partial specific ...
... means of the relation Uh = ( 1 / po ) ( 55 ) In general the hydrodynamic partial specific volume is not identical to the partial specific volume determined by conventional means ( see Part C of this series ) . The partial specific ...
Page 93
... means of a photomultiplier which mechanically scans the image of the ultracen- trifuge cell . The logarithm of the photocurrent is plotted by means of a pen recorder . The system can be operated in a double - beam manner to eliminate ...
... means of a photomultiplier which mechanically scans the image of the ultracen- trifuge cell . The logarithm of the photocurrent is plotted by means of a pen recorder . The system can be operated in a double - beam manner to eliminate ...
Page 94
... means of an air - turbine drive which has the advantage of simplicity and freedom from vibration compared with the electric motor gearbox combination . Rotor speeds of up to 60,000 rpm are possible , giving a maximum centrifugal force ...
... means of an air - turbine drive which has the advantage of simplicity and freedom from vibration compared with the electric motor gearbox combination . Rotor speeds of up to 60,000 rpm are possible , giving a maximum centrifugal force ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone