Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 53
... mixture . Albright and Williams ( 1967 ) have also given methods for the extrapolation of Mapp values of heterogeneous nonideal solutions . 3. Average Molecular Weights Other than M The z average molecular weight of a protein mixture M2 ...
... mixture . Albright and Williams ( 1967 ) have also given methods for the extrapolation of Mapp values of heterogeneous nonideal solutions . 3. Average Molecular Weights Other than M The z average molecular weight of a protein mixture M2 ...
Page 59
... mixture , the M ° values obtained at various concentrations must be ex- trapolated to zero concentration of solute and the same problems exist as for sedimentation equilibrium . 5. The Detection of Heterogeneity For an ideal homogeneous ...
... mixture , the M ° values obtained at various concentrations must be ex- trapolated to zero concentration of solute and the same problems exist as for sedimentation equilibrium . 5. The Detection of Heterogeneity For an ideal homogeneous ...
Page 174
... mixture of the two proteins , under interacting conditions ; ( 2 ) each protein individ- ually in the same solvent . The latter experiments give M2 , M4 , B22 and ẞ44 . Substitution of these values into Eq . ( 18 ) together with the ...
... mixture of the two proteins , under interacting conditions ; ( 2 ) each protein individ- ually in the same solvent . The latter experiments give M2 , M4 , B22 and ẞ44 . Substitution of these values into Eq . ( 18 ) together with the ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone