## Physical principles and techniques of protein chemistry, Volume 2 |

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Page 54

The high speed method (Yphantis, 1964) can yield the number-average

Effects of Nonideality Globular proteins near their isoelectric points and in

buffered solutions ...

The high speed method (Yphantis, 1964) can yield the number-average

**molecular weight**as well as the Mw and Mz values of the original solution. E.Effects of Nonideality Globular proteins near their isoelectric points and in

buffered solutions ...

Page 67

For example, if we consider a solute that self- associates to form dimers, the

weight-average

d(x2) plot will refer only to the concentration that exists in the cell at the point at

which ...

For example, if we consider a solute that self- associates to form dimers, the

weight-average

**molecular weight**that can be calculated from the slope of a d ln j/d(x2) plot will refer only to the concentration that exists in the cell at the point at

which ...

Page 159

J (11) Factoring out IdMi and dividing both sides by KC2 = Kid, we have Re ZCtMj

(, ft2 -LCjMjR\\ . i£C2 " 2C, V 3 SC^ / U^aj -W„-»»„^g* (12b) We see that, in a

polydisperse system, light scattering yields a weight average

and ...

J (11) Factoring out IdMi and dividing both sides by KC2 = Kid, we have Re ZCtMj

(, ft2 -LCjMjR\\ . i£C2 " 2C, V 3 SC^ / U^aj -W„-»»„^g* (12b) We see that, in a

polydisperse system, light scattering yields a weight average

**molecular weight**and ...

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### Contents

Ultracentrifugal Analysis H Coates Glossary of Symbols | 3 |

Fundamentals of the Method | 5 |

Light Scattering | 12 |

Copyright | |

40 other sections not shown

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### Common terms and phrases

absorption acid amino atoms axial ratio axis band beam binding Biol Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant denaturation density gradient dependence determined diffusion dilution Doty effect ellipsoid enzyme equation experiments extrapolation field Fraser frequency fringe groups Gurd histidine hydrogen increase instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering linear macromolecule magnetic maximum measured meniscus method molecular weight molecule myoglobin Natl nuclei observed obtained optical density optical system parameters partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons radius random coil Rayleigh reference refractive index relaxation residues resonance RNase rods rotation rotor sample schlieren Section sector sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero Zimm zone