Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
From inside the book
Results 1-3 of 60
Page 48
... necessary to fill the cells precisely to known levels using a precision syringe or microm- eter syringe . This is necessary to ensure that the solution column is always very slightly shorter than the solvent column as shown in Fig . 16 ...
... necessary to fill the cells precisely to known levels using a precision syringe or microm- eter syringe . This is necessary to ensure that the solution column is always very slightly shorter than the solvent column as shown in Fig . 16 ...
Page 56
... NECESSARY FOR A RELIABLE RESULT 1. Selection of a Suitable Concentration of Solute and Speed of Centrifuge A concentration of about 15 mg / ml ( in a 12 - mm cell ) is optimal for a precise measurement of the schlieren trace . To ensure ...
... NECESSARY FOR A RELIABLE RESULT 1. Selection of a Suitable Concentration of Solute and Speed of Centrifuge A concentration of about 15 mg / ml ( in a 12 - mm cell ) is optimal for a precise measurement of the schlieren trace . To ensure ...
Page 81
... necessary to produce a detectable displacement of the biphasic equilib- rium schlieren pattern at equilibrium , a knowledge of the variance of the equilibrium protein distribution is necessary . In principle this may be obtained by ...
... necessary to produce a detectable displacement of the biphasic equilib- rium schlieren pattern at equilibrium , a knowledge of the variance of the equilibrium protein distribution is necessary . In principle this may be obtained by ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
51 other sections not shown
Other editions - View all
Common terms and phrases
absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone