Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 73
... negative density gradient of the protein solute is proportional to the initial protein concentration and is greatest during the first minutes of the run . After 15 minutes the maximum negative density gradient for the hypothetical ...
... negative density gradient of the protein solute is proportional to the initial protein concentration and is greatest during the first minutes of the run . After 15 minutes the maximum negative density gradient for the hypothetical ...
Page 127
... negative charges , are placed in aqueous solution at low or high pH , there is a considerable excess of positive or negative charge on the molecule and it expands on dilution . However , in the region of the isoelectric pH ( the pH at ...
... negative charges , are placed in aqueous solution at low or high pH , there is a considerable excess of positive or negative charge on the molecule and it expands on dilution . However , in the region of the isoelectric pH ( the pH at ...
Page 165
... Negative values of 2 дтз 2 . ( age ) ag2 / T.P.3 " and ac3 aC2 / T.P.3 mean negative preferential binding of component 3 to component 2 , or an excess of principal solvent in the immediate vicinity of the protein mole- cules ( Ullman ...
... Negative values of 2 дтз 2 . ( age ) ag2 / T.P.3 " and ac3 aC2 / T.P.3 mean negative preferential binding of component 3 to component 2 , or an excess of principal solvent in the immediate vicinity of the protein mole- cules ( Ullman ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone