Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 22
... plate on the photographic plate . In use , the metal line at the phase border is set at an angle to the image of the source slit . At all points in the ultracentrifuge cell where there are positive refrac- tive index gradients , the ...
... plate on the photographic plate . In use , the metal line at the phase border is set at an angle to the image of the source slit . At all points in the ultracentrifuge cell where there are positive refrac- tive index gradients , the ...
Page 31
... plate . The plate is aligned on the measuring microscope by the use of " reference " fringes that appear at each end of the pattern , and which are generated by inter- ference between rays of light passing through pairs of slits in the ...
... plate . The plate is aligned on the measuring microscope by the use of " reference " fringes that appear at each end of the pattern , and which are generated by inter- ference between rays of light passing through pairs of slits in the ...
Page 50
... plate . The baseline correction plate is measured at identical X positions to the equilibrium plate and the displacements of the buffer - buffer fringes relative to the reference fringes are expressed as fractions of a fringe and used ...
... plate . The baseline correction plate is measured at identical X positions to the equilibrium plate and the displacements of the buffer - buffer fringes relative to the reference fringes are expressed as fractions of a fringe and used ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone