## Physical Principles and Techniques of Protein Chemistry, Part 2Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |

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Page 89

The biphasic

to give the concentration distribution through the zone expressed in arbitrary

units . The natural logarithms of concentration values for a series of points evenly

...

The biphasic

**plot**obtained from enlarging the schlieren photograph is integratedto give the concentration distribution through the zone expressed in arbitrary

units . The natural logarithms of concentration values for a series of points evenly

...

Page 156

+ 16 * sina ( 012 ) 4 : 1 ) ( 10 ) This is the well - known Zimm ( 1948a )

equation . For this

between 25° and 145° ) at different concentrations . The points are plotted as a ...

+ 16 * sina ( 012 ) 4 : 1 ) ( 10 ) This is the well - known Zimm ( 1948a )

**plot**equation . For this

**plot**, the scattering is measured as a function of angle ( usuallybetween 25° and 145° ) at different concentrations . The points are plotted as a ...

Page 173

Then , the limiting slope of a

the slope of the

values of all the higher ki ' s are obtained from such a sequence of

Then , the limiting slope of a

**plot**of Mw / XMm as a function of XC / Mm gives ka ;the slope of the

**plot**of ( - 4k XC / Mm ) as a function of ( XC / Mm ) 2 gives kz ; thevalues of all the higher ki ' s are obtained from such a sequence of

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### Contents

LIST OF CONTRIBUTORS | 1 |

Zonal Analytical Velocity Sedimentation | 2 |

Light Scattering | 12 |

Copyright | |

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absorption acid applied associated average band beam binding boundary calculated cell centrifugal Chem chemical shifts column complex component concentration constant correction density density gradient dependence described determined diffusion distance distribution effect equal equation equilibrium error et al experiments factor field frequency fringe function give given gradient groups increase interaction length light macromolecule magnetic maximum means measured meniscus method molecular weight molecule necessary observed obtained occur optical particle pattern peak photographic Phys plate plot Polymer position possible procedure protein protein solution protons range ratio reference refractive region relatively resonance rotation rotor salt sample scattering schlieren sector sedimentation sedimentation coefficient shape shown shows single slit solution solvent specific spectra spectrum speed structure studies technique temperature tion ultracentrifuge usually values viscosity volume zero zone