Physical Principles and Techniques of Protein Chemistry, Part 2Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 137
However , light scattering and sedimentation studies showed clearly that side - by
- side aggregation occurs , and hence the increase in viscosity was attributed to
higher aggregates produced by the shearing stress in the viscometer .
However , light scattering and sedimentation studies showed clearly that side - by
- side aggregation occurs , and hence the increase in viscosity was attributed to
higher aggregates produced by the shearing stress in the viscometer .
Page 139
Application of this model has produced estimates of the persistence length of 800
A ( Moha et al . , 1964 ) and 1100 A ( Tsvetkov et al . , 1965 ) for PBLG in N , N ' -
formdimethylamide and of 260 A ( Applequist and Doty , 1962 ) for poly - e ...
Application of this model has produced estimates of the persistence length of 800
A ( Moha et al . , 1964 ) and 1100 A ( Tsvetkov et al . , 1965 ) for PBLG in N , N ' -
formdimethylamide and of 260 A ( Applequist and Doty , 1962 ) for poly - e ...
Page 233
In the IR 12 , for example , the attenuation produced by a series of settings of the
trimmer comb can be measured to + 0 . 002 transmittance using single beam
mode and used to check or calibrate the photometer . Alternatively , a high speed
...
In the IR 12 , for example , the attenuation produced by a series of settings of the
trimmer comb can be measured to + 0 . 002 transmittance using single beam
mode and used to check or calibrate the photometer . Alternatively , a high speed
...
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Contents
LIST OF CONTRIBUTORS | 1 |
Zonal Analytical Velocity Sedimentation | 2 |
Light Scattering | 12 |
Copyright | |
19 other sections not shown
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absorption acid applied associated average band beam binding boundary calculated cell centrifugal Chem chemical shifts column complex component concentration constant correction density density gradient dependence described determined diffusion distance distribution effect equal equation equilibrium error et al experiments factor field frequency fringe function give given gradient groups increase interaction length light macromolecule magnetic maximum means measured meniscus method molecular weight molecule necessary observed obtained occur optical particle pattern peak photographic Phys plate plot Polymer position possible procedure protein protein solution protons range ratio reference refractive region relatively resonance rotation rotor salt sample scattering schlieren sector sedimentation sedimentation coefficient shape shown shows single slit solution solvent specific spectra spectrum speed structure studies technique temperature tion ultracentrifuge usually values viscosity volume zero zone