Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 171
... reflect intermolecular attractions and repulsions . All of these effects will be reflected in the second virial coefficient , i.e. , in the term ( μ2 ( e ) / C2 ) г , p of Eqs . ( 17 ) and ( 25 ) . The concentration dependence of the ...
... reflect intermolecular attractions and repulsions . All of these effects will be reflected in the second virial coefficient , i.e. , in the term ( μ2 ( e ) / C2 ) г , p of Eqs . ( 17 ) and ( 25 ) . The concentration dependence of the ...
Page 306
... reflect the titration curves of the acids . Thus the change in the shift for the Ca protons from 0 to 0.85 ppm corresponds to the transi- tion from cation to dipolar ion and the change from 0.85 to 1.27 ppm to the transition from ...
... reflect the titration curves of the acids . Thus the change in the shift for the Ca protons from 0 to 0.85 ppm corresponds to the transi- tion from cation to dipolar ion and the change from 0.85 to 1.27 ppm to the transition from ...
Page 380
... reflect not simply the degree of steric hindrance by neighboring structures but also may result from an abnormal pKa . Fur- thermore , the difference between the titration behavior of harbor seal and sperm whale myoglobins ( Hartzell et ...
... reflect not simply the degree of steric hindrance by neighboring structures but also may result from an abnormal pKa . Fur- thermore , the difference between the titration behavior of harbor seal and sperm whale myoglobins ( Hartzell et ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone