Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 374
... yields the concentration of the bound ion . Dividing this value by the total protein concentration in the inside solution yields . The most common shortcom- ing of this method in practice results from failure to achieve equilibrium ...
... yields the concentration of the bound ion . Dividing this value by the total protein concentration in the inside solution yields . The most common shortcom- ing of this method in practice results from failure to achieve equilibrium ...
Page 378
... yield a product of composition with respect to histidine derivatives of 5.3 residues of histidine , 4.5 of dicarboxymethyl histidine , 1.2 of 3 - carboxymethyl histidine , and 0.9 of 1 - carboxy- methyl histidine . The preponderant ...
... yield a product of composition with respect to histidine derivatives of 5.3 residues of histidine , 4.5 of dicarboxymethyl histidine , 1.2 of 3 - carboxymethyl histidine , and 0.9 of 1 - carboxy- methyl histidine . The preponderant ...
Page 399
... yield very detailed information on which to base the comparisons . By analogy with what is known of similar complexes ( Freeman , 1967 ; Gurd and Bryce , 1966 ; Bryce , 1966 ) it is probable that the four nitrogen . atoms specified ...
... yield very detailed information on which to base the comparisons . By analogy with what is known of similar complexes ( Freeman , 1967 ; Gurd and Bryce , 1966 ; Bryce , 1966 ) it is probable that the four nitrogen . atoms specified ...
Contents
Ultracentrifugal Analysis | 1 |
J H Coates Glossary of Symbols 23435 37 | 2 |
Fundamentals of the Method | 5 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone