Molecular Cloning: A Laboratory Manual, Volume 1The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. |
From inside the book
Results 1-5 of 9
... Nucleic Acids and Oligonucleotides Gene or cDNA fragment of interest Media Induction medium 1x MOPS salts ( for recipe , please see Buffers and Solutions above ) 0.2 % ( w / v ) glucose 0.2 % ( w / v ) casamino acids ( vitamin assay ...
... Nucleic Acids Res . 13 : 1923-1938 . Burgess R.R. 1996. Purification of overproduced Escherichia coli RNA polymerase sigma factors by solubilizing inclusion bodies and refolding from Sarkosyl . Methods Enzymol . 273 : 145-149 . Calos ...
... Nucleic Acids Res . 8 : 1893-1912 . Grantham R. , Gautier C. , Gouy M. , Jacobzone M. , and Mercier R. 1981. Codon catalog usage is a genome strategy modulated for gene expressivity . Nucleic Acids Res . 9 : r43-174 . Grantham R ...
... Nucleic Acids Res . 13 : 1151–1162 . Norrander J. , Kempe T. , and Messing J. 1983. Construction of improved M13 vectors using oligodeoxynucleotide - directed mutagenesis . Gene 26 : 101-106 . Nossal N.G. and Heppel L.E. 1966. The ...
... nucleic acids into cultured cells . The work of Graham and van der Eb ( 1973 ) on transforma- tion of mammalian cells by viral DNAs in the presence of calcium phosphate laid the foundation for the biochemical transformation of ...
Contents
15-3 | |
15-12 | |
15-49 | |
15-55 | |
15-64 | |
16-6 | |
16-7 | |
16-12 | |
Chapter 12 | 12 |
Chapter 9 | 11 |
1 | 13 |
In Vitro Amplification of DNA by the Polymerase 8 1 | 8 |
Preparation of Radiolabeled DNA and RNA Probes 9 1 | 9 |
INTRODUCTION | 3 |
Chapter 4 | 4 |
PROTOCOLS | 16 |
16-13 | |
16-21 | |
16-43 | |
17-1 | |
17-3 | |
17-5 | |
Transcriptional Runon Assays 17 23 | 17-23 |
INFORMATION PANELS | 17-75 |
INFORMATION PANELS | 17-91 |
INFORMATION PANELS | 17-99 |
Chapter 18 | 18-3 |
Extraction Purification and Analysis of mRNA from 7 1 | 18-7 |
Chapter 10 | 18-10 |
Chapter 14 | 18-14 |
INFORMATION PANELS | 18-82 |
INFORMATION PANELS | 18-107 |
INFORMATION PANELS | 18-115 |
Appendices | 18-137 |
Chapter 13 | 13 |
1 | 30 |
INFORMATION PANELS | 6 |
Chapter 8 | 8 |
Working with Highcapacity Vectors 4 1 | 4 |
INTRODUCTION | 10 |
DNA Sequencing 12 1 | 12 |
Working with Synthetic Oligonucleotide Probes 10 1 | 10 |
INTRODUCTION | R-20 |
1 | i |
PREFACE XV | xv |
Chapter 2 | 2 |
Screening Expression Libraries 14 1 | 14 |
1 | 56 |
Chapter 4 | 59 |
7 | 75 |
11 | 84 |
INFORMATION PANELS | 109 |
Amplifiers | 144 |
AnalogtoDigital Converters | 155 |
References | 165 |
CALIBRATION AND RESPONSE 93 | 173 |
577 | 183 |
59599 | 203 |