Molecular Cloning: A Laboratory Manual, Volume 1The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. |
From inside the book
Results 1-5 of 7
... fluorescent protein was cloned from Aequorea victoria , a jellyfish found in abun- dance in Puget Sound , Washington State . This picture of a 50 - mm medusa was taken on color film by flash photography and shows light reflected from ...
... fluorescent protein , whose expression is driven by a pan - specific promoter such as the human cytomegalovirus immediate early gene region promoter and enhancer . Tracer plasmids of this kind are available from many different ...
... fluorescent protein ( please see the information panel on GREEN FLUORESCENT PROTEIN in Chapter 17 ) . These can be purchased from several commercial manufacturers ( e.g. , pCMV - SPORT - B - gal , Life Technologies , or PEGFP - F ...
... fluorescent protein , a farnesylated enhanced GFP ( EGFP - F ) that remains bound to the plasma membrane in both ... fluorescence microscopy . ( Adapted , with permission , from CLONTECH . ) TABLE 16-4 Some Lipids Used in Lipofection ...
... fluorescent protein , and ẞ - glu- curonidase ( for plants ) or selectable markers such as neomycin resistance . Plasmids expressing a gene or CDNA of interest can be used after the process has been optimized . Investigators debate ...
Contents
15-3 | |
15-12 | |
15-49 | |
15-55 | |
15-64 | |
16-6 | |
16-7 | |
16-12 | |
Chapter 12 | 12 |
Chapter 9 | 11 |
1 | 13 |
In Vitro Amplification of DNA by the Polymerase 8 1 | 8 |
Preparation of Radiolabeled DNA and RNA Probes 9 1 | 9 |
INTRODUCTION | 3 |
Chapter 4 | 4 |
PROTOCOLS | 16 |
16-13 | |
16-21 | |
16-43 | |
17-1 | |
17-3 | |
17-5 | |
Transcriptional Runon Assays 17 23 | 17-23 |
INFORMATION PANELS | 17-75 |
INFORMATION PANELS | 17-91 |
INFORMATION PANELS | 17-99 |
Chapter 18 | 18-3 |
Extraction Purification and Analysis of mRNA from 7 1 | 18-7 |
Chapter 10 | 18-10 |
Chapter 14 | 18-14 |
INFORMATION PANELS | 18-82 |
INFORMATION PANELS | 18-107 |
INFORMATION PANELS | 18-115 |
Appendices | 18-137 |
Chapter 13 | 13 |
1 | 30 |
INFORMATION PANELS | 6 |
Chapter 8 | 8 |
Working with Highcapacity Vectors 4 1 | 4 |
INTRODUCTION | 10 |
DNA Sequencing 12 1 | 12 |
Working with Synthetic Oligonucleotide Probes 10 1 | 10 |
INTRODUCTION | R-20 |
1 | i |
PREFACE XV | xv |
Chapter 2 | 2 |
Screening Expression Libraries 14 1 | 14 |
1 | 56 |
Chapter 4 | 59 |
7 | 75 |
11 | 84 |
INFORMATION PANELS | 109 |
Amplifiers | 144 |
AnalogtoDigital Converters | 155 |
References | 165 |
CALIBRATION AND RESPONSE 93 | 173 |
577 | 183 |
59599 | 203 |