Molecular Cloning: A Laboratory Manual, Volume 1The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. |
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Results 1-5 of 8
... tetracycline ( 0.1 μg / ml ) at sublethal concentrations is based on observations by Lee and Beckwith ( 1986 ) , who noted that many suppressors of secretory pathway defects ( sec mutants ) mapped in genes encoding protein synthesis com ...
... tetracycline - regulated promoter ( Gossen and Bujard 1992 ; Gossen et al . 1995 ; Shockett et al . 1995 ) , or an ecdysone - regulated system ( No et al . 1996 ) . Alternatively , conditional alleles of some genes can be constructed ...
... tetracycline - resistant transformants is ~ 100 - fold less than the number of ampicillin - resistant transformants ( Steele et al . 1994 ) . This effect is not seen when the plasmid is introduced to the bacteria by the calcium chloride ...
... tetracycline - responsive promoters . Proc . Natl . Acad . Sci . 89 : 5547-5551 . Gossen M. , Freundlieb S. , Bender G. , Muller G. , Hillen W. , and Bujard H. 1995. Transcriptional activation by tetracyclines in mammalian cells ...
... tetracycline - regulated system provides autoregu- latory , inducible gene expression in cultured cells and trans- genic mice . Proc . Natl . Acad . Sci . 92 : 6522-6526 . Simonsen C.C. and Levinson A.D. 1983. Isolation and expression ...
Contents
15-3 | |
15-12 | |
15-49 | |
15-55 | |
15-64 | |
16-6 | |
16-7 | |
16-12 | |
Chapter 12 | 12 |
Chapter 9 | 11 |
1 | 13 |
In Vitro Amplification of DNA by the Polymerase 8 1 | 8 |
Preparation of Radiolabeled DNA and RNA Probes 9 1 | 9 |
INTRODUCTION | 3 |
Chapter 4 | 4 |
PROTOCOLS | 16 |
16-13 | |
16-21 | |
16-43 | |
17-1 | |
17-3 | |
17-5 | |
Transcriptional Runon Assays 17 23 | 17-23 |
INFORMATION PANELS | 17-75 |
INFORMATION PANELS | 17-91 |
INFORMATION PANELS | 17-99 |
Chapter 18 | 18-3 |
Extraction Purification and Analysis of mRNA from 7 1 | 18-7 |
Chapter 10 | 18-10 |
Chapter 14 | 18-14 |
INFORMATION PANELS | 18-82 |
INFORMATION PANELS | 18-107 |
INFORMATION PANELS | 18-115 |
Appendices | 18-137 |
Chapter 13 | 13 |
1 | 30 |
INFORMATION PANELS | 6 |
Chapter 8 | 8 |
Working with Highcapacity Vectors 4 1 | 4 |
INTRODUCTION | 10 |
DNA Sequencing 12 1 | 12 |
Working with Synthetic Oligonucleotide Probes 10 1 | 10 |
INTRODUCTION | R-20 |
1 | i |
PREFACE XV | xv |
Chapter 2 | 2 |
Screening Expression Libraries 14 1 | 14 |
1 | 56 |
Chapter 4 | 59 |
7 | 75 |
11 | 84 |
INFORMATION PANELS | 109 |
Amplifiers | 144 |
AnalogtoDigital Converters | 155 |
References | 165 |
CALIBRATION AND RESPONSE 93 | 173 |
577 | 183 |
59599 | 203 |