Cell ElectrophoresisThis book presents a summary of the application and instrumentation of cell electrophoresis. The method of making cell purification and characterization possible according to the cellular negative surface charge density is discussed, and ideas for future developments are explained. The negative electrostatic forces at cell surfaces provide information about cell-cell interaction, blood vessel sealing, cytokine actions, cell transformation, ion transport phenomena, and other biological phenomena. Recalculations of the physical principles of cell electrophoresis reveal possibilities for removing disruptive factors caused by electrical current, heat, and sedimentation. The introduction of computer technology, the performance of simultaneous two-parameter measurements, and the application of cell-friendly but current-inert buffer systems render the method more reliable and efficient. |
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Contents
Chapter | 3 |
Chapter | 12 |
Numerical Description of Zone Electrophoresis in the Continuous Flow | 33 |
Chapter 3 | 51 |
Theoretical and Experimental Studies on the Stabilization of Hydrodynamic | 57 |
Chapter 4 | 75 |
Chapter 5 | 103 |
Cell Electrophoresis Using Antibodies and Antigens as Ligands | 113 |
Chapter 10 | 199 |
Chapter 9 | 200 |
Chapter 11 | 219 |
Chapter 12 | 241 |
Chapter 13 | 255 |
Chapter 14 | 267 |
Chapter 15 | 283 |
Chapter 16 | 315 |
Chapter 7 | 145 |
Chapter 8 | 163 |
Electrophoresis of Cells at a Physiological Ionic Strength 181 | 318 |
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Common terms and phrases
acid adhesion adsorption antibody antibody treatment antigen B-cells Bauer Biol biological Biophys Ca2+ cancer cation cell electrophoresis cell fractions cell separation cell surface cells per milliliter cm²/Vs conductivity continuous flow electrophoresis culture decrease density gradient dextran distribution effect electric charge electric field electrode electrohydrodynamic electrokinetic electrolyte electroosmosis electroosmotic electrophoresis system electrophoretic mobility endothelial cells EPM values Equation erythrocytes experiments Figure flight fluid forces free-flow electrophoresis function glycocalyx Hannig HEK cells human Immunol increase incubation interactions ionic strength ions isoelectric layer low ionic strength lymphocytes measured medium membrane method microelectrophoresis microgravity migration mJ/m² molecules monocytes NaCl negative surface charge parameters particles phoresis polar polymer proteins Q buffer rabbit receptor repulsion resolution sample stream Schütt sedimentation separation chamber Slivinsky solution space studies surface charge surface charge density temperature thickness tumor cells velocity zeta potential zone zwitterion