Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 117
... Section VI , A , and the ques- tion of rigidity of rods is considered in Section VI , E , 1 . The intrinsic viscosity of a model which consists of a rigid string of identical , spherical beads has been shown by Kirkwood and Auer ( 1951 ) ...
... Section VI , A , and the ques- tion of rigidity of rods is considered in Section VI , E , 1 . The intrinsic viscosity of a model which consists of a rigid string of identical , spherical beads has been shown by Kirkwood and Auer ( 1951 ) ...
Page 123
... Section IV , A ) , i.e. , a = 0. Thus the intrinsic viscosity of various globular proteins ranging from ribonuclease ( M = 13,700 ) to hemoglobin ( M = 67,000 ) falls within the range 0.031 to 0.043 dl / gm , with no correlation between ...
... Section IV , A ) , i.e. , a = 0. Thus the intrinsic viscosity of various globular proteins ranging from ribonuclease ( M = 13,700 ) to hemoglobin ( M = 67,000 ) falls within the range 0.031 to 0.043 dl / gm , with no correlation between ...
Page 231
... section is accurately known relative to the surface of the specimen in contact with the nylon block . Interference fringes sometimes appear in the spectra of good quality sections and these can be eliminated by the method described in ...
... section is accurately known relative to the surface of the specimen in contact with the nylon block . Interference fringes sometimes appear in the spectra of good quality sections and these can be eliminated by the method described in ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone