Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 3
... addition of detergent or other substances such as urea . Changes in the state of aggregation of proteins as a result of such procedures may be conveniently followed in the velocity ultracentrifuge and the molecular weight of the ...
... addition of detergent or other substances such as urea . Changes in the state of aggregation of proteins as a result of such procedures may be conveniently followed in the velocity ultracentrifuge and the molecular weight of the ...
Page 79
... addition of fairly high concentrations of low molecular weight solute to the solution ; sucrose , deuterium oxide , sodium chloride , and cesium chloride have all been used . Under the influence of a high centrif- ugal field , the ...
... addition of fairly high concentrations of low molecular weight solute to the solution ; sucrose , deuterium oxide , sodium chloride , and cesium chloride have all been used . Under the influence of a high centrif- ugal field , the ...
Page 190
... addition , and laying the Teflon stopper on a dust - free surface during the addition , 18 it should be possible to make five or six consecutive additions of protein stock to the cell before picking up the inevitable dust particle , or ...
... addition , and laying the Teflon stopper on a dust - free surface during the addition , 18 it should be possible to make five or six consecutive additions of protein stock to the cell before picking up the inevitable dust particle , or ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone