Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 2
... coefficient sedimentation coefficient of zero concentration Svedberg unit distance from center of rotation concentration angular velocity of rotation time from effective start of sedimentation molecular weight v partial specific volume ...
... coefficient sedimentation coefficient of zero concentration Svedberg unit distance from center of rotation concentration angular velocity of rotation time from effective start of sedimentation molecular weight v partial specific volume ...
Page 13
... coefficient may be ex- panded thus ( Goldberg , 1953 ) In yı = BM11 + and combining Eqs . ( 5 ) and ( 7 ) we obtain ... coefficient . It is in fact equal to twice the B coefficient in the second term of the virial expansion in which the ...
... coefficient may be ex- panded thus ( Goldberg , 1953 ) In yı = BM11 + and combining Eqs . ( 5 ) and ( 7 ) we obtain ... coefficient . It is in fact equal to twice the B coefficient in the second term of the virial expansion in which the ...
Page 43
... COEFFICIENT MEASUREMENTS 1. Concentration Dependence of Sedimentation Coefficients If the S20 , w value for a protein is found to increase with concentration , it can usually be inferred that the solute is an interacting system ...
... COEFFICIENT MEASUREMENTS 1. Concentration Dependence of Sedimentation Coefficients If the S20 , w value for a protein is found to increase with concentration , it can usually be inferred that the solute is an interacting system ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone