Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 175
... curve obtained by adding calculated values of the progressive ionization term to the short dashed line . The lower ... curve of the protein ( see Kirkwood and Timasheff , 1956 ) ; after correction for this term , the data may be plotted ...
... curve obtained by adding calculated values of the progressive ionization term to the short dashed line . The lower ... curve of the protein ( see Kirkwood and Timasheff , 1956 ) ; after correction for this term , the data may be plotted ...
Page 307
... curve correspond to the titration of the C. amino and carboxyl groups , while the C2H curve reflects the protonation of N1 . The C , H2 and C.H curves reflect the titration of all ionizable groups , to different extents ( Mc- Donald and ...
... curve correspond to the titration of the C. amino and carboxyl groups , while the C2H curve reflects the protonation of N1 . The C , H2 and C.H curves reflect the titration of all ionizable groups , to different extents ( Mc- Donald and ...
Page 458
... curve varies with the collagen derivative , but it can be measured accurately . It is possible , moreover , to estimate the heat of transition . Consequently , it has been suggested that the melting curves obtained with differential ...
... curve varies with the collagen derivative , but it can be measured accurately . It is possible , moreover , to estimate the heat of transition . Consequently , it has been suggested that the melting curves obtained with differential ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone