Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 134
... DENATURATION STUDIES The process of denaturation involves a drastic change in one or more of the properties of the protein in solution as a result of the addition of particular solutes , including acid or base , the effect of heat or ...
... DENATURATION STUDIES The process of denaturation involves a drastic change in one or more of the properties of the protein in solution as a result of the addition of particular solutes , including acid or base , the effect of heat or ...
Page 422
... denaturation by hydrogen ions in terms of the stabilization of the denatured form by protonation of the previously inaccessible imidazole groups masked in their conjugate base form ( Breslow and Gurd , 1962 ; Clark and Gurd , 1967 ...
... denaturation by hydrogen ions in terms of the stabilization of the denatured form by protonation of the previously inaccessible imidazole groups masked in their conjugate base form ( Breslow and Gurd , 1962 ; Clark and Gurd , 1967 ...
Page 459
... denaturation ( Steim , 1965 ) . Differential thermal analyses of six different protein preparations indicate that denaturation in solution can be recognized on the thermograms as an endotherm with a characteristic contour . For the ...
... denaturation ( Steim , 1965 ) . Differential thermal analyses of six different protein preparations indicate that denaturation in solution can be recognized on the thermograms as an endotherm with a characteristic contour . For the ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone